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Journal / Bioengineering / Mobil Tek Moleküllü FRET Problarının Otomatik İki …
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Mobil Tek Moleküllü FRET Problarının Otomatik İki Boyutlu Mekansal Analizi
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Mobil Tek Moleküllü FRET Problarının Otomatik İki Boyutlu Mekansal Analizi

Article DOI: 10.3791/63124-v • 08:26 min • November 23rd, 2021
November 23rd, 2021 •
1, 2, 2, 2, 1
1Institute of Applied Physics, TU Wien, 2Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna

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Bu makalede, geniş alan floresan mikroskopisi kullanılarak mobil, tek moleküllü Förster rezonans enerji transferi (smFRET) tabanlı probların mekansal analizi için bir yöntem sunulmaktadır. Yeni geliştirilen yazılım araç seti, doğru FRET verimliliği ve moleküler konumlar da dahil olmak üzere hareketli probların smFRET zaman izlerinin zamanın işlevleri olarak belirlenmesini sağlar.

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