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Isolamento de células endoteliais da retina murinas para sequenciamento de próxima geração
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Isolamento de células endoteliais da retina murinas para sequenciamento de próxima geração

Article DOI: 10.3791/63133-v • 09:59 min • October 11th, 2021
October 11th, 2021 •
1,2, 1, 2,3,4, 1,2,4,5
1Department of Cell Biology, University of Virginia School of Medicine, 2Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine, 3Department of Cardiology, University of Virginia School of Medicine, 4Hematovascular Biology Center, University of Virginia School of Medicine, 5Yale Cardiovascular Research Center, Yale University School of Medicine

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Este protocolo descreve um método para o isolamento de células endoteliais da retina pós-natais murinas otimizado para o rendimento, pureza e viabilidade celular. Essas células são adequadas para abordagens de sequenciamento de próxima geração.

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Biologia do Desenvolvimento Edição 176 células endoteliais desenvolvimento vascular vascularização murina da retina sequenciamento de próxima geração isolamento celular primário viabilidade pureza
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