JoVE Journal
Biology
Biology
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인간 혈액-뇌 장벽을 모델링하는 삼중 배양 세포 시스템
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Article
DOI:
10.3791/63134-v
•
09:21 min
•
November 30th, 2021
인간 혈액-뇌 장벽을 모델링하는 삼중 배양 세포 시스템
November 30th, 2021
•
Eleonora Rizzi*1, Clémence Deligne*1, Lucie Dehouck1, Roberta Bilardo2, Yasutero Sano3, Fumitaka Shimizu3, Takashi Kanda3, Marina Resmini2, Fabien Gosselet1, Marie-Pierre Dehouck1, Caroline Mysiorek1
1Univ. Artois, UR 2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), 2Queen Mary University of London, Department of Chemistry, 3Yamaguchi University Graduate School of Medicine, Department of Neurology and Clinical Neuroscience
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
이 프로토콜은 인간 혈액-뇌 장벽(BBB) 시험관내 모델을 확립하는 방법을 설명합니다. 내피 세포와 혈관주위세포는 삽입 필터의 양쪽(혈액 구획)에 파종되고 성상세포는 하단 웰(뇌 구획)에 파종됩니다. 상기 모델 특성화는 나노입자 수송 실험에 사용되었다.