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Journal / Bioengineering / A Simple Microfluidic Chip for Long-Term Growth…
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JoVE Journal Bioengineering
A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans
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A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans

Article DOI: 10.3791/63136-v • 10:45 min • April 11th, 2022
April 11th, 2022 •
1,2,3,4, 1,5, 2
1National Centre for Biological Sciences, TIFR, 2Department of Biological Sciences, TIFR, 3InSTEM, 4Manipal Academy of Higher Education, 5Department of Mechanical Engineering, The University of Texas at Austin

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Summary
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The protocol describes a simple microfluidic chip design and microfabrication methodology used to grow C. elegans in presence of a continuous food supply for up to 36 h. The growth and imaging device also enables intermittent long-term high-resolution imaging of cellular and sub-cellular processes during development for several days.

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Microfluidic Chip Caenorhabditis Elegans Long-term Growth Imaging Device High Resolution Imaging Reuse Cleanliness Fabricate Dust-free Devices Leakage Design Pattern Flow Layer Control Layer Rectangular Shapes Photo Masks Laser Plotter Polyester Based Film Silicon Wafers Potassium Hydroxide Deionized Water Compressed Nitrogen Gas Hot Plate Spin Coder Hexamethyldisilane Photoresist Thickness
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