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Bioengineering

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JoVE Journal Bioengineering
En simpel mikrofluidisk chip til langsigtet vækst og billeddannelse af Caenorhabditis elegans
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En simpel mikrofluidisk chip til langsigtet vækst og billeddannelse af Caenorhabditis elegans

Article DOI: 10.3791/63136-v • 10:45 min • April 11th, 2022
April 11th, 2022 •
1,2,3,4, 1,5, 2
1National Centre for Biological Sciences, TIFR, 2Department of Biological Sciences, TIFR, 3InSTEM, 4Manipal Academy of Higher Education, 5Department of Mechanical Engineering, The University of Texas at Austin

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Protokollen beskriver et simpelt mikrofluidisk chipdesign og mikrofabrikationsmetode, der bruges til at dyrke C. elegans i nærvær af en kontinuerlig fødevareforsyning i op til 36 timer. Vækst- og billeddannelsesenheden muliggør også intermitterende langsigtet højopløsningsbilleddannelse af cellulære og subcellulære processer under udvikling i flere dage.

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Bioengineering udgave 182 C. elegans mikrofluidisk chip on-chip vækst langsigtet billeddannelse neuronal billeddannelse vulva celle afstamning
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