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JoVE Journal Bioengineering
Un semplice chip microfluidico per la crescita a lungo termine e l'imaging di Caenorhabditis elegans
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Un semplice chip microfluidico per la crescita a lungo termine e l'imaging di Caenorhabditis elegans

Article DOI: 10.3791/63136-v • 10:45 min • April 11th, 2022
April 11th, 2022 •
1,2,3,4, 1,5, 2
1National Centre for Biological Sciences, TIFR, 2Department of Biological Sciences, TIFR, 3InSTEM, 4Manipal Academy of Higher Education, 5Department of Mechanical Engineering, The University of Texas at Austin

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Il protocollo descrive una semplice progettazione di chip microfluidici e una metodologia di microfabbricazione utilizzata per coltivare C. elegans in presenza di una fornitura continua di cibo fino a 36 ore. Il dispositivo di crescita e imaging consente inoltre l'imaging intermittente ad alta risoluzione a lungo termine dei processi cellulari e subcellulari durante lo sviluppo per diversi giorni.

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Bioingegneria Numero 182 C. elegans chip microfluidico crescita su chip imaging a lungo termine imaging neuronale linea cellulare vulva
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