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JoVE Journal
Immunology and Infection

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使用捕获测定法检测基于病毒样颗粒(VLP)的疫苗上显示的抗原中和敏感表位
 
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使用捕获测定法检测基于病毒样颗粒(VLP)的疫苗上显示的抗原中和敏感表位

Article DOI: 10.3791/63137-v 05:15 min February 10th, 2022
February 10th, 2022

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Summary

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Click here for the English version.

在这里,我们提出了一种检测抗原显示病毒样颗粒(VLP)的中和表位的方案。使用包膜糖蛋白特异性单克隆抗体偶联至蛋白G偶联磁珠进行人免疫缺陷病毒(HIV)衍生VLP的免疫沉淀。随后使用病毒核心蛋白Gag特异性抗体对捕获的VLP进行SDS-PAGE和蛋白质印迹分析。

Tags

免疫学和感染,第180期,免疫沉淀,磁珠,抗原显示,中和表位,病毒样颗粒,抗体
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