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JoVE Journal
Developmental Biology

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Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5
 
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Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5

Article DOI: 10.3791/63148-v 10:49 min February 14th, 2022
February 14th, 2022

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Summary

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In diesem Protokoll beschreiben wir, wie die Amnionhöhle der Maus bei E7.5 mit Lentivirus injiziert wird, was zu einer gleichmäßigen Transduktion der gesamten Neuralplatte führt, mit minimalen nachteiligen Auswirkungen auf das Überleben oder die Embryonalentwicklung.

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Developmental Biology Ausgabe 180
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