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使用活细胞显微镜测量扩散过程中的细胞边缘突起动力学
JoVE Journal
Biology
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JoVE Journal Biology
Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy
DOI:

05:50 min

November 01, 2021

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Chapters

  • 00:04Introduction
  • 00:47Glass-Bottom Dish Coating
  • 01:48Preparation of Cells for Imaging
  • 02:27Microscope Setup and Imaging
  • 03:23Image Analysis
  • 04:29Results: Cell-Edge Protrusion Assay at the Edge of Spreading Cells
  • 05:08Conclusion

Summary

Automatic Translation

该协议旨在测量扩散细胞边缘突起的动态参数(突起,缩回,褶皱)。

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