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使用活细胞显微镜测量扩散过程中的细胞边缘突起动力学
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Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
使用活细胞显微镜测量扩散过程中的细胞边缘突起动力学
DOI:
10.3791/63157-v
•
05:50 min
•
November 01, 2021
•
Nikola Lukic*
1
,
Trishna Saha*
1
,
Stefanie Lapetina
,
Michal Gendler
,
Gilad Lehmann
,
Anthony J. Koleske
3
,
Hava Gil-Henn
1
The Azrieli Faculty of Medicine
,
Bar-Ilan University
,
2
Department of Molecular Biophysics and Biochemistry
,
Yale University
,
3
Department of Neuroscience
,
Yale University
Chapters
00:04
Introduction
00:47
Glass-Bottom Dish Coating
01:48
Preparation of Cells for Imaging
02:27
Microscope Setup and Imaging
03:23
Image Analysis
04:29
Results: Cell-Edge Protrusion Assay at the Edge of Spreading Cells
05:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议旨在测量扩散细胞边缘突起的动态参数(突起,缩回,褶皱)。
Tags
Cell-edge Protrusion
Cell Migration
Live-cell Microscopy
Cell Motility
Fibronectin
BSA
Trypsin
Hemacytometer
Phase Contrast Microscopy
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