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JoVE Journal Developmental Biology
Eks Utero Kultur av museembryoer fra pregastrkulering til avansert organogenese
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Eks Utero Kultur av museembryoer fra pregastrkulering til avansert organogenese

Article DOI: 10.3791/63160-v • 07:14 min • October 19th, 2021
October 19th, 2021 •
1, 1
1Department of Molecular Genetics, Weizmann Institute of Science

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En forbedret plattform for hel-embryo kultur tillater kontinuerlig og robust ex utero utvikling av postimplantasjon mus embryoer i opptil seks dager, fra pregastrulation stadier til avansert organogenese. I denne protokollen beskriver vi standardprosedyren for vellykket embryokultur ved hjelp av statiske plater og roterende flaskesystemer.

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Utviklingsbiologi Utgave 176 Museembryo Ex Utero Kultur Gastrulation Organogenese Embryogenese Pattedyr utvikling
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