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Journal / Biochemistry / Syntese af Masarimycin, en lille molekylehæmmer…
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Biochemistry

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JoVE Journal Biochemistry
Syntese af Masarimycin, en lille molekylehæmmer af grampositiv bakterievækst
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Syntese af Masarimycin, en lille molekylehæmmer af grampositiv bakterievækst

Article DOI: 10.3791/63191-v • 09:10 min • January 7th, 2022
January 7th, 2022 •
1, 1, 1,2
1Department of Science and Technology, Bryant University, 2Center for Health and Behavioral Sciences, Bryant University

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En detaljeret protokol præsenteres til fremstilling af det bakteriostatiske diamid masarimycin, en lille molekylesonde, der hæmmer væksten af Bacillus subtilis og Streptococcus pneumoniae ved at målrette cellevægnedbrydning. Dens anvendelse som en kemisk sonde er påvist i synergi / antagonisme assays og morfologiske undersøgelser med B. subtilis og S. pneumoniae.

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Biokemi udgave 179 Peptidoglycan metabolisme autolysin kemisk biologi hæmmer cellevæg
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