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JoVE Journal Genetics
ACT1-CUP1-Assays bestimmen die substratspezifischen Sensitivitäten von spleißeosomalen Mutanten in knospender Hefe
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ACT1-CUP1-Assays bestimmen die substratspezifischen Sensitivitäten von spleißeosomalen Mutanten in knospender Hefe

Article DOI: 10.3791/63232-v • 07:31 min • June 30th, 2022
June 30th, 2022 •
1
1College of Arts and Sciences, Northwest University

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Der ACT1-CUP1-Assay, ein Kupferwachstumsassay, ermöglicht eine schnelle Ablesung des Precursor-Messenger-RNA-Spleißens (pre-mRNA) und des Einflusses mutierter Spleißfaktoren auf die spleißöosomale Funktion. Diese Studie bietet ein Protokoll und hebt die Anpassungsmöglichkeiten hervor, um die interessierende Spleißfrage zu beantworten.

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Genetics pre-mRNA splicing Saccharomyces cerevisiae Spliceosom ACT1-CUP1 Growth Assay genetic screen mutant screen non-consensus sequence
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