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Journal / Genetics / ACT1-CUP1 Testleri, Tomurcuklanan Mayadaki…
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Genetics

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JoVE Journal Genetics
ACT1-CUP1 Testleri, Tomurcuklanan Mayadaki Splisozomal Mutantların Substrata Özgü Hassasiyetlerini Belirler
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ACT1-CUP1 Testleri, Tomurcuklanan Mayadaki Splisozomal Mutantların Substrata Özgü Hassasiyetlerini Belirler

Article DOI: 10.3791/63232-v • 07:31 min • June 30th, 2022
June 30th, 2022 •
1
1College of Arts and Sciences, Northwest University

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Bir bakır büyüme testi olan ACT1-CUP1 testi, öncü mesajcı RNA (pre-mRNA) eklemesinin ve mutant ekleme faktörlerinin spleozomal fonksiyon üzerindeki etkisinin hızlı bir şekilde okunmasını sağlar. Bu çalışma bir protokol sağlar ve ilgilenilen ekleme sorusunu ele almak için mümkün olan özelleştirmeyi vurgular.

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Genetik Sayı 184 pre-mRNA ekleme Saccharomyces cerevisiae spliceosome ACT1-CUP1 büyüme testi genetik tarama mutant ekran konsensüssüz sekans
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