JoVE Monthly Highlights: April 2019

1, 1
1Journal of Visualized Experiments

Multiscale Sampling of a Heterogeneous Water/Metal Catalyst Interface using Density Functional Theory and Force-Field Molecular Dynamics

Cameron J. Bodenschatz*1, Xiaohong Zhang*1, Tianjun Xie*1, Jeremy Arvay1,2, Sapna Sarupria1, Rachel B. Getman1
1Department of Chemical and Biomolecular Engineering, Clemson University, 2Davidson School of Chemical Engineering, Purdue University
* These authors contributed equally

The goal of the protocol presented here is to generate and sample trajectories of configurations of liquid water molecules around catalytic species on a flat transition metal surface. The sampled configurations can be used as starting structures in quantum mechanics-based methods.

Intense Pulsed Light for the Treatment of Dry Eye Owing to Meibomian Gland Dysfunction

Luca Vigo1, Giuseppe Giannaccare2, Stefano Sebastiani2, Marco Pellegrini2, Francesco Carones1
1Carones Ophthalmology Center, 2Ophthalmology Unit, S.Orsola-Malpighi University Hospital, DIMES, University of Bologna

Dry eye disease is an increasingly common condition, which strongly impair patients' life quality. Recently, a new device employing intense pulsed light, specifically designed for the periocular area, has been shown to improve tear film stability and ocular discomfort symptoms in dry eye disease owing to meibomian gland dysfunction.

Radio Frequency Magnetron Sputtering of GdBa2Cu3O7−δ/ La0.67Sr0.33MnO3 Quasi-bilayer Films on SrTiO3 (STO) Single-crystal Substrates

Ying Wang1,2, Zhen Li1,2, YongSheng Liu1, Yijie Li2, Linfei Liu2, Da Xu2, Xiaojing Luo1, Tian Gao1,3, Yanyan Zhu1,4, Luozeng Zhou3, Jianming Xu3
1Department of Physics, Mathematics, Shanghai Key Laboratory of Materials Protection and Advanced Materials in Electric Power, Shanghai University of Electric Power, 2Key Laboratory of Artificial Structure and Quantum Control, Ministry of Education, Department of Physics, Shanghai Jiao Tong University, 3Shanghai Institute of Space Power-sources, 4Shanghai Key Laboratory of High Temperature Superconductors, Shanghai University

Here, we present a protocol to grow LSMO nanoparticles and (Gd) BCO films on (001) SrTiO3 (STO) single-crystal substrates by radio frequency (RF)-sputtering.

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

Yena Kim1,2, Ji Hyeon Ju1,2,3
1CiSTEM Laboratory, Catholic Induced Pluripotent Stem Cell (iPSC) Research Center, College of Medicine, The Catholic University of Korea, 2Department of Biomedicine & Health Science, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 3Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea

We propose a protocol that shows how to differentiate induced pluripotent stem cell-derived keratinocytes and fibroblasts and generate a 3D skin organoid, using these keratinocytes and fibroblasts. This protocol contains an additional step of generating a humanized mice model. The technique presented here will improve dermatologic research.

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