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JoVE Journal Biology
Functionele beoordeling van kinesine-7 CENP-E in spermatocyten met behulp van in vivo remming, immunofluorescentie en flowcytometrie
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Functionele beoordeling van kinesine-7 CENP-E in spermatocyten met behulp van in vivo remming, immunofluorescentie en flowcytometrie

Article DOI: 10.3791/63271-v • 09:41 min • December 28th, 2021
December 28th, 2021 •
1,2, 1,2, 3,4, 1,2, 1,2, 1,2, 1,2, 1,2
1Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, 3Fujian Obstetrics and Gynecology Hospital, 4Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University

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Dit artikel meldt een in vivo remming van CENP-E door middel van abdominale chirurgie en testiculaire injectie van GSK923295, een waardevol model voor mannelijke meiotische deling. Met behulp van de immunofluorescentie, flowcytometrie en transmissie-elektronenmicroscopietests tonen we aan dat CENP-E-remming resulteert in chromosoomafwijking en genoominstabiliteit in spermatocyten van muizen.

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Deze maand in JoVE nummer 178 kinesine spermatocyt meiose CENP-E spindel chromosoom microtubuli
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