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Journal / Biology / in vivo阻害、免疫蛍光法およびフローサイトメトリーを用いた精母細胞におけるキネシン-7…
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JoVE Journal Biology
in vivo阻害、免疫蛍光法およびフローサイトメトリーを用いた精母細胞におけるキネシン-7 CENP-Eの機能評価(英語)
Reverse Genetic Approach to Identify Regulators of Pigmentation using Zebrafish
Journal (Biology)
Reverse Genetic Approach to Identify Regulators of Pigmentation using Zebrafish
Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
Journal (Biology)
Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
 
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in vivo阻害、免疫蛍光法およびフローサイトメトリーを用いた精母細胞におけるキネシン-7 CENP-Eの機能評価(英語)

Article DOI: 10.3791/63271-v • 09:41 min • December 28th, 2021
December 28th, 2021 •
1,2, 1,2, 3,4, 1,2, 1,2, 1,2, 1,2, 1,2
1Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, 3Fujian Obstetrics and Gynecology Hospital, 4Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University

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本稿では,男性減数分裂の貴重なモデルであるGSK923295の腹部手術と精巣注射によるCENP-Eの in vivo 阻害について報告する.免疫蛍光法、フローサイトメトリー法、透過型電子顕微鏡アッセイを用いて、CENP-E阻害がマウス精母細胞に染色体のミスアライメントとゲノム不安定性をもたらすことを示しています。

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今月のJoVE、第178号、キネシン、精母細胞、減数分裂、CENP-E、紡錘体、染色体、微小管
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