Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Isolation and Characterization of Intact…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Isolation and Characterization of Intact Phycobilisome in Cyanobacteria
Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy
Journal (Biology)
Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy
Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential
Journal (Biology)
Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential
 

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria

Article DOI: 10.3791/63272-v • 06:26 min • November 10th, 2021
November 10th, 2021 •
1, 1,2
1Department of Life Science, National Taiwan University, 2Institute of Plant Biology, National Taiwan University

Chapters

Summary
Automatic Translation

The current protocol details the isolation of phycobilisomes from cyanobacteria by centrifugation through a discontinuous sucrose density gradient. The fractions of intact phycobilisomes are confirmed by 77K fluorescent emission spectrum and SDS-PAGE analysis. The resulting phycobilisome fractions are suitable for negative staining of TEM and mass spectrometry analysis.

Tags

Isolation Characterization Intact Phycobilisome Cyanobacteria Protocol Technique Small Scale Reliable Efficient Method Cell Culture OD At 750 Nanometers B-HEPES Medium Constant Stirring 30 Degree Celsius Exponential Growth Phase Centrifuge Supernatant Cell Pellets Potassium Phosphate Buffer PH Seven Wet Weight Electronic Balance Cell Suspension Glass Beads Bead Beater
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter