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Subculture and Cryopreservation of Esophageal Adenocarcinoma Organoids: Pros and Cons for Single Cell Digestion
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Cancer Research
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JoVE Journal Cancer Research
Subculture and Cryopreservation of Esophageal Adenocarcinoma Organoids: Pros and Cons for Single Cell Digestion

Subculture and Cryopreservation of Esophageal Adenocarcinoma Organoids: Pros and Cons for Single Cell Digestion

DOI:
10.3791/63281-v

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10:42 min

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July 06, 2022

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, , , , ,
1Department of General, Visceral, Cancer and Transplantation Surgery,University Hospital Cologne, 2Institute of Pathology,University Hospital Cologne

Chapters

  • 00:05Introduction
  • 00:53Preparations in Advance
  • 01:23Harvesting Organoids
  • 03:05Subculturing without Digestion
  • 04:23Subculturing with Single-Cell Digestion
  • 06:48Cryopreservation of the Undigested and Single Cell Digested PDOs
  • 08:08Results: Observation and Identification of Cell Culture
  • 09:46Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the methods of subculture and cryopreservation of esophageal adenocarcinoma organoids with and without single cell digestion to enable researchers to choose appropriate strategies based on their experimental design.

Tags

Keywords: Esophageal Adenocarcinoma OrganoidsSubcultureSingle-cell DigestionPDOsECM GelCell Recovery SolutionCentrifugationCryopreservation

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