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JoVE Journal Neuroscience
Protocolo Baseado em Microdissecção a Laser para a Análise LC-MS/MS do Perfil Proteômico de Grânulos de Neuromelanina
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Protocolo Baseado em Microdissecção a Laser para a Análise LC-MS/MS do Perfil Proteômico de Grânulos de Neuromelanina

Article DOI: 10.3791/63289-v • 07:35 min • December 16th, 2021
December 16th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2, 3,4, 5, 1,2, *1,2, *1,2
1Medizinisches Proteom-Center, Medical Faculty, Ruhr-University Bochum, 2Medical Proteome Analysis, Center for Proteindiagnostics (PRODI), Ruhr-University Bochum, 3Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy, University Hospital Wuerzburg, 4Psychiatry Department of Clinical Research, University of Southern Denmark Odense University Hospital, 5Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, University Hospital of Wuerzburg, University of Wuerzburg
* These authors contributed equally

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Um protocolo robusto é apresentado aqui para isolar grânulos de neuromelanina de tecido humano post-mortem substantia nigra pars compacta via microdissecção a laser. Este protocolo revisado e otimizado minimiza massivamente o tempo necessário para a coleta de amostras, reduz a quantidade de amostra necessária e melhora a identificação e quantificação de proteínas pela análise LC-MS/MS.

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Neurociência Edição 178 Grânulos de neuromelanina microdissecção a laser espectrometria de massa substantia nigra pars compacta monitoramento de reações paralelas aquisição dependente de dados
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