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JoVE Journal Developmental Biology
Visualisering av Cytoskeleton-avhengig smugling av lipidholdige organeller i Drosophila embryoer
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Visualisering av Cytoskeleton-avhengig smugling av lipidholdige organeller i Drosophila embryoer

Article DOI: 10.3791/63291-v • 08:55 min • December 13th, 2021
December 13th, 2021 •
1, 1
1Department of Biology, University of Rochester

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I det tidlige Drosophila-embryoet er mange organeller motile. I prinsippet kan de avbildes levende via spesifikke fluorescerende sonder, men eggeskallet forhindrer direkte påføring av embryoet. Denne protokollen beskriver hvordan man introduserer slike sonder via mikroinjeksjon, og deretter analyserer bulk organelle bevegelse via partikkelbilde velocimetry.

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Utviklingsbiologi utgave 178
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