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Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice
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Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice

Article DOI: 10.3791/63293-v • 07:34 min • December 16th, 2021
December 16th, 2021 •
1, 1, 1
1Department of Microbiology and Immunology, University of Texas Medical Branch

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Summary
Automatic Translation

In this protocol, a gut microbiota antigen-specific T cell adoptive transfer colitis model is described. CD4+ T cells are isolated from CBir1 TCR transgenic mice. These are specific for an immunodominant gut microbiota antigen CBir1 flagellin, which is transferred into recipient Rag1-/- mice, leading to intestinal inflammation.

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Intestinal Inflammation Adoptive Transfer CBir1 TCR Transgenic CD4+ T Cells Immunodeficient Mice Inflammatory Bowel Disease IBD Colitis Model Gut Bacteria Antigen T-cell Responses Euthanizing Abdominal Incision Skin Abdominal Muscle Tissue Spleen Removal Culture Dish Washing Buffer Cell Suspension Cell Strainer Tris Ammonium Chloride Lysis Buffer
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