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Journal / Medicine / CBir1 TCR Transgenik CD4+ T Hücrelerinin İmmün…
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JoVE Journal Medicine
CBir1 TCR Transgenik CD4+ T Hücrelerinin İmmün Yetmezlikçi Farelere Benimsenen Transferi ile Bağırsak İltihabının İndüksiyonu
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CBir1 TCR Transgenik CD4+ T Hücrelerinin İmmün Yetmezlikçi Farelere Benimsenen Transferi ile Bağırsak İltihabının İndüksiyonu

Article DOI: 10.3791/63293-v • 07:34 min • December 16th, 2021
December 16th, 2021 •
1, 1, 1
1Department of Microbiology and Immunology, University of Texas Medical Branch

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Bu protokolde bağırsak mikrobiyota antijenine özgü T hücreli benimseyen transfer kolit modeli tanımlanmıştır. CD4+ T hücreleri CBir1 TCR transgenik farelerden izole edilmiştir. Bunlar, alıcı Rag1/- farelere aktarılan ve bağırsak iltihabına yol açan immünodominant bağırsak mikrobiyota antijeni CBir1 flagellin için özeldir.

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