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JoVE Journal Developmental Biology
Differensiering av humane pluripotente stamceller i betacelleforløpere i bukspyttkjertelen i et 2D-kultursystem
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Differensiering av humane pluripotente stamceller i betacelleforløpere i bukspyttkjertelen i et 2D-kultursystem

Article DOI: 10.3791/63298-v • 10:12 min • December 16th, 2021
December 16th, 2021 •
1,2, 1,2
1College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Qatar Foundation, 2Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF)

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Denne protokollen beskriver en forbedret metode for å øke kouttrykket av PDX1- og NKX6.1-transkripsjonsfaktorer i pankreasprogenitorer avledet fra humane pluripotente stamceller (hPSCer) i plane monolag. Dette oppnås ved å fylle opp den friske matrisen, manipulere celletettheten og dissosiere de endodermale cellene.

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Utviklingsbiologi utgave 178
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