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转录组水平上人类原发性肠系膜动脉内皮细胞的分离和分析
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Article
DOI:
10.3791/63307-v
•
09:45 min
•
March 14th, 2022
转录组水平上人类原发性肠系膜动脉内皮细胞的分离和分析
March 14th, 2022
•
Naseeb Kaur Malhi*1, Yingjun Luo*1, Xiaofang Tang*1, Kiran Sriram1,3, Riccardo Calandrelli2, Sheng Zhong2, Zhen Bouman Chen1,3
1Department of Diabetes Complications and Metabolism, City of Hope, 2Department of Bioengineering, University of California San Diego, 3Irell and Manella Graduate School of Biological Sciences, City of Hope
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
该方案描述了从人肠系膜动脉中分离、培养和分析内皮细胞。此外,还提供了一种制备用于空间转录组学的人类动脉的方法。蛋白质组学,转录组学和功能测定可以在分离的细胞上进行。该方案可以重新用于任何中型或大型动脉。