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Isolering og profilering av humane primære mesenteriske arterielle endotelceller på transkripsjonsnivå
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Isolering og profilering av humane primære mesenteriske arterielle endotelceller på transkripsjonsnivå

Article DOI: 10.3791/63307-v • 09:45 min • March 14th, 2022
March 14th, 2022 •
*1, *1, *1, 1,3, 2, 2, 1,3
1Department of Diabetes Complications and Metabolism, City of Hope, 2Department of Bioengineering, University of California San Diego, 3Irell and Manella Graduate School of Biological Sciences, City of Hope
* These authors contributed equally

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Protokollen beskriver isolasjon, kultur og profilering av endotelceller fra human mesenterisk arterie. I tillegg er det gitt en metode for å forberede menneskelig arterie for romlig transkripsjon. Proteomikk, transkripsjon og funksjonelle analyser kan utføres på isolerte celler. Denne protokollen kan gjenbrukes for alle middels eller store arteri.

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