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Aislamiento y perfil de células endoteliales arteriales mesentéricas primarias humanas a nivel de transcriptoma
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Aislamiento y perfil de células endoteliales arteriales mesentéricas primarias humanas a nivel de transcriptoma

Article DOI: 10.3791/63307-v • 09:45 min • March 14th, 2022
March 14th, 2022 •
*1, *1, *1, 1,3, 2, 2, 1,3
1Department of Diabetes Complications and Metabolism, City of Hope, 2Department of Bioengineering, University of California San Diego, 3Irell and Manella Graduate School of Biological Sciences, City of Hope
* These authors contributed equally

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El protocolo describe el aislamiento, el cultivo y el perfil de las células endoteliales de la arteria mesentérica humana. Además, se proporciona un método para preparar la arteria humana para la transcriptómica espacial. La proteómica, la transcriptómica y los ensayos funcionales se pueden realizar en células aisladas. Este protocolo se puede reutilizar para cualquier arteria de tamaño mediano o grande.

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Medicina Número 181
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