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Journal / Bioengineering / Residu-specifieke uitwisseling van Proline door…
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Bioengineering

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JoVE Journal Bioengineering
Residu-specifieke uitwisseling van Proline door Proline-analogen in fluorescerende eiwitten
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Residu-specifieke uitwisseling van Proline door Proline-analogen in fluorescerende eiwitten: hoe "moleculaire chirurgie" van de ruggengraat de vouwing en stabiliteit beïnvloedt

Article DOI: 10.3791/63320-v • 10:31 min • February 3rd, 2022
February 3rd, 2022 •
1, 2, 3, 1,2, 1
1Institute of Chemistry, Technische Universität Berlin, 2Department of Chemistry, University of Manitoba, 3Institute of Physics, Martin-Luther-Universität Halle-Wittenberg

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Om de beperkingen van klassieke site-directed mutagenese te overwinnen, werden proline analogen met specifieke modificaties opgenomen in verschillende fluorescerende eiwitten. We laten zien hoe de vervanging van waterstof door fluor of van de enkele door dubbele bindingen in prolineresiduen ("moleculaire chirurgie") fundamentele eiwiteigenschappen beïnvloedt, inclusief hun vouwing en interactie met licht.

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Bio-engineering niet-canonieke aminozuren proline analogen selectieve drukincorporatie eiwitvouwing vouwkinetiek eiwitstabiliteit
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