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JoVE Journal Chemistry
Mangesidet massespektrometrisk undersøgelse af neuropeptider i Callinectes sapidus
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Mangesidet massespektrometrisk undersøgelse af neuropeptider i Callinectes sapidus

Article DOI: 10.3791/63322-v • 09:22 min • May 31st, 2022
May 31st, 2022 •
*1, *1, 1,2
1Department of Chemistry, University of Wisconsin-Madison, 2School of Pharmacy, University of Wisconsin-Madison
* These authors contributed equally

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Massespektrometrisk karakterisering af neuropeptider giver sekvens-, kvantations- og lokaliseringsinformation. Denne optimerede arbejdsgang er ikke kun nyttig til neuropeptidundersøgelser, men også andre endogene peptider. Protokollerne her beskriver prøveforberedelse, MS-erhvervelse, MS-analyse og databasegenerering af neuropeptider ved hjælp af LC-ESI-MS, MALDI-MS-spotting og MALDI-MS-billeddannelse.

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Kemi udgave 183 neuropeptid massespektrometri peptidrensning isotopmærkning de novo-sekventering kvantificering LC MALDI massespektrometribilleddannelse databasesøgning transkriptomminedrift
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