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Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid

Article DOI: 10.3791/63326-v • 10:53 min • January 20th, 2022
January 20th, 2022 •
1,2, 1,2, 3, 1,2
1Center for Cancer Biology, VIB (BE), 2Department of Oncology, KU Leuven (BE), 3Netherlands Cancer Institute, Department of Molecular Pathology, Oncode Institute (NL)

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Summary
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This protocol describes a novel mammary imaging window with a replaceable lid (R.MIW). Intravital microscopy after implantation of the R.MIW allows for longitudinal and multi-day imaging of the healthy and diseased mammary gland with a cellular resolution during the different developmental stages.

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Longitudinal Intravital Microscopy Mammary Imaging Window Replaceable Lid Cellular Dynamics Tissue Rearrangements Healthy Mammary Gland Diseased Mammary Gland Longitudinal Intravital Imaging Optimal Imaging Conditions Tissue Manipulation Drug Administration Mammary Tumor Initiation Mammary Tumor Progression Single-cell Level Cyanoacrylate Adhesive Glue Glass Cover Slip Airtight Seal Disinfecting Lids Acetone
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