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JoVE Journal Neuroscience
Måling af egenskaber for membranens periodiske skelet i Axon Initial Segment ved hjælp af 3D-struktureret belysningsmikroskopi (3D-SIM)
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Måling af egenskaber for membranens periodiske skelet i Axon Initial Segment ved hjælp af 3D-struktureret belysningsmikroskopi (3D-SIM)

Article DOI: 10.3791/63327-v • 07:40 min • February 11th, 2022
February 11th, 2022 •
1, 2, 1,3,4
1Minerva Foundation Institute for Medical Research, 2Department of Computer Science, Aalto University School of Science, 3HiLIFE - Neuroscience Center, University of Helsinki, 4HiLIFE - Institute of Biotechnology, University of Helsinki

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Den foreliggende protokol beskriver en metode til at visualisere og måle actinringe og andre komponenter i membranens periodiske skelet af axon-initialsegmentet ved hjælp af dyrkede rotte hippocampale neuroner og 3D-struktureret belysningsmikroskopi (3D-SIM).

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