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JoVE Journal Bioengineering
Incapsulamento rapido del citoscheletro ricostituito all'interno di vescicole unilamellari giganti
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Incapsulamento rapido del citoscheletro ricostituito all'interno di vescicole unilamellari giganti

Article DOI: 10.3791/63332-v • 07:48 min • November 10th, 2021
November 10th, 2021 •
*1, *1, 2, 3, 1,4,5,6
1Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences, Harvard University, 3Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, 5Department of Biophysics, University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program, University of Michigan, Ann Arbor
* These authors contributed equally

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Questo articolo introduce un metodo semplice per la produzione rapida di vescicole unilamellari giganti con proteine citoscheletriche incapsulate. Il metodo si rivela utile per la ricostituzione bottom-up delle strutture citoscheletriche nelle interazioni confinamento e citoscheletro-membrana.

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Bioingegneria Numero 177 Trasferimento di emulsione cDICE GUV ricostituzione bottom-up actina fascina
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