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Journal / Biochemistry / Overvågning af proteinaggregeringskinetik in vivo …
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Biochemistry

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JoVE Journal Biochemistry
Overvågning af proteinaggregeringskinetik in vivo ved hjælp af automatiseret inklusionstælling i caenorhabditis elegans
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Overvågning af proteinaggregeringskinetik in vivo ved hjælp af automatiseret inklusionstælling i caenorhabditis elegans

Article DOI: 10.3791/63365-v • 06:49 min • December 17th, 2021
December 17th, 2021 •
*1, *1, *1, 1
1Bijvoet Centre for Biomolecular Research, Utrecht University
* These authors contributed equally

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Her præsenteres en metode til analyse af proteinaggregeringskinetik i nematoden Caenorhabditis elegans. Dyr fra en alderssynkroniseret population afbildes på forskellige tidspunkter, efterfulgt af semiautomatiseret inklusionstælling i CellProfiler og tilpasning til en matematisk model i AmyloFit.

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