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Journal / Biochemistry / Overvåking Protein Aggregation Kinetics In Vivo…
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Biochemistry

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JoVE Journal Biochemistry
Overvåking Protein Aggregation Kinetics In Vivo ved hjelp av automatisert inkluderingstelling i Caenorhabditis elegans
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Overvåking Protein Aggregation Kinetics In Vivo ved hjelp av automatisert inkluderingstelling i Caenorhabditis elegans

Article DOI: 10.3791/63365-v • 06:49 min • December 17th, 2021
December 17th, 2021 •
*1, *1, *1, 1
1Bijvoet Centre for Biomolecular Research, Utrecht University
* These authors contributed equally

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Her presenteres en metode for analyse av proteinaggregeringskinetikk i nematoden Caenorhabditis elegans. Dyr fra en alderssynkronisert populasjon er avbildet på forskjellige tidspunkter, etterfulgt av halvautomatisert inkluderingstelling i CellProfiler og tilpasning til en matematisk modell i AmyloFit.

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