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Journal / Biochemistry / Samtidig visualisering af dynamikken i tværbundne …
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Biochemistry

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JoVE Journal Biochemistry
Samtidig visualisering af dynamikken i tværbundne og enkelte mikrotubuli in vitro ved hjælp af TIRF-mikroskopi
Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues
Journal (Biochemistry)
Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues
Dissection of Human Retina and RPE-Choroid for Proteomic Analysis
Journal (Biochemistry)
Dissection of Human Retina and RPE-Choroid for Proteomic Analysis
 
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Samtidig visualisering af dynamikken i tværbundne og enkelte mikrotubuli in vitro ved hjælp af TIRF-mikroskopi

Article DOI: 10.3791/63377-v • 07:20 min • February 18th, 2022
February 18th, 2022 •
*1,2, *1, 1,2
1Molecular Biology, Massachusetts General Hospital, 2Department of Genetics, Harvard Medical School
* These authors contributed equally

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Her præsenteres et TIRF-mikroskopibaseret in vitro-rekonstitutionsassay for samtidig at kvantificere og sammenligne dynamikken i to mikrotubulipopulationer. En metode er beskrevet til samtidig at se den kollektive aktivitet af flere mikrotubuli-associerede proteiner på tværbundne mikrotubuli bundter og enkelte mikrotubuli.

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