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Journal / Cancer Research / Gepoolde shRNA-bibliotheekscreening om factoren…
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JoVE Journal Cancer Research
Gepoolde shRNA-bibliotheekscreening om factoren te identificeren die een fenotype van medicijnresistentie moduleren
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Gepoolde shRNA-bibliotheekscreening om factoren te identificeren die een fenotype van medicijnresistentie moduleren

Article DOI: 10.3791/63383-v • 14:51 min • June 17th, 2022
June 17th, 2022 •
1,2, 1,2, 2,3, 2,3, 1,2,3, 1,2,3
1Department of Clinical Haematology, Christian Medical College, 2Department of Biotechnology, Thiruvalluvar University, 3Centre for Stem Cell Research, Christian Medical College

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High-throughput RNA-interferentie (RNAi) screening met behulp van een pool van lentivirale shRNA's kan een hulpmiddel zijn om therapeutisch relevante synthetische dodelijke doelwitten bij maligniteiten te detecteren. We bieden een gepoolde shRNA-screeningsbenadering om de epigenetische effectoren bij acute myeloïde leukemie (AML) te onderzoeken.

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Kankeronderzoek High-throughput screening RNAi screening shRNA epigenetische factor cytarabine AraC resistentie
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