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JoVE Journal Neuroscience
En tyngdekraftsmatet transkardiasjonsperfusjonsmetode for histologisk analyse av musens sentralnervesystem
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En tyngdekraftsmatet transkardiasjonsperfusjonsmetode for histologisk analyse av musens sentralnervesystem

Article DOI: 10.3791/63386-v • 10:37 min • January 21st, 2022
January 21st, 2022 •
1, 2, 1,3
1Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, 2Department of Neurology, State University of New York Upstate Medical University, 3Department of Neuroscience and Physiology, State University of New York Upstate Medical University

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En praktisk tyngdekraftmatet perfusjonsmetode for histologisk analyse av musens sentralnervesystem presenteres. Immunfluoreserende påvisning av fosforylatert α-synuklein er demonstrert i en musemodell av Parkinsons sykdom. Dette arbeidet beskriver også omfattende transkardiell perfusjon, disseksjon, vevsfrysing / innebygging og frosne seksjoneringstrinn.

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