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Journal / Biochemistry / En robust kryo-elektronmikroskopi (cryo-EM)…
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Biochemistry

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JoVE Journal Biochemistry
En robust kryo-elektronmikroskopi (cryo-EM) prosesseringsarbeidsflyt med kryoSPARC, RELION og scipion
Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
Journal (Biochemistry)
Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
Journal (Biochemistry)
Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
 
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En robust kryo-elektronmikroskopi (cryo-EM) prosesseringsarbeidsflyt med kryoSPARC, RELION og scipion

Article DOI: 10.3791/63387-v • 13:43 min • January 31st, 2022
January 31st, 2022 •
1, 1,2
1Institute for Quantitative Biomedicine, Rutgers University, 2Department of Biochemistry & Microbiology, Rutgers University

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Denne artikkelen beskriver hvordan du effektivt bruker tre cryo-EM-prosesseringsplattformer, det vil si cryoSPARC v3, RELION-3 og Scipion 3, for å lage en enkelt og robust arbeidsflyt som gjelder for en rekke enkeltpartikkeldatasett for høyoppløselig strukturbestemmelse.

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Biokjemi Utgave 179 kryo-elektronmikroskopi kryo-EM enkeltpartikkelanalyse SPA kryoSPARC RELION Scipion bildebehandling strukturberegning AAV
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