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Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing
Preparation of Working Solution and Semen Sample for Leukocyte Analysis (Video) | JoVE
Journal (Medicine)
Preparation of Working Solution and Semen Sample for Leukocyte Analysis (Video) | JoVE
A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice
Journal (Medicine)
A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice
 
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Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing

Article DOI: 10.3791/63397-v • 10:44 min • March 23rd, 2022
March 23rd, 2022 •
1,2, 1, 1,2, 1, 2, 1, 3, 1,2
1Department of Anatomy & Embryology, Leiden University Medical Center, 2Department of Cardiology, Leiden University Medical Center, 3Department of Medical Statistics and Bioinformatics, Leiden University Medical Center

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Dit protocol beschrijft de gedetailleerde, low-input monstervoorbereiding voor single-nucleus sequencing, inclusief de dissectie van muis superieure cervicale en stellate ganglia, celdissociatie, cryopreservatie, nucleus isolatie en hashtag barcoding.

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Geneeskunde Nummer 181
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