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Låginmatning av kärnisolering och multiplexering med streckkodade antikroppar av mussympatiska ganglier för enkärnig RNA-sekvensering
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Låginmatning av kärnisolering och multiplexering med streckkodade antikroppar av mussympatiska ganglier för enkärnig RNA-sekvensering

Article DOI: 10.3791/63397-v • 10:44 min • March 23rd, 2022
March 23rd, 2022 •
1,2, 1, 1,2, 1, 2, 1, 3, 1,2
1Department of Anatomy & Embryology, Leiden University Medical Center, 2Department of Cardiology, Leiden University Medical Center, 3Department of Medical Statistics and Bioinformatics, Leiden University Medical Center

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Detta protokoll beskriver den detaljerade, låginmatningsprovberedningen för enkärnig sekvensering, inklusive dissektion av musöverlägsna livmoderhals- och stellatganglier, celldissociation, kryokonservering, kärnisolering och hashtag-streckkodning.

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Medicin utgåva 181
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