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Journal / Bioengineering / Biofabbricazione assistita da microscopia…
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Bioengineering

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JoVE Journal Bioengineering
Biofabbricazione assistita da microscopia dell'ossigeno a prezzi accessibili di sferoidi multicellulari
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Biofabbricazione assistita da microscopia dell'ossigeno a prezzi accessibili di sferoidi multicellulari

Article DOI: 10.3791/63403-v • 13:21 min • April 6th, 2022
April 6th, 2022 •
*1, *1, 2, 3, 1
1Tissue Engineering and Biomaterials Group, Department of Human Structure and Repair, Faculty of Medical and Health Sciences, Ghent University, 2Health and Happiness (H&H) Group, National Food Innovation Hub, 3Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology
* These authors contributed equally

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Il protocollo descrive la generazione di sferoidi ad alto rendimento per la bioprinting utilizzando l'analisi multiparametrica della loro ossigenazione e morte cellulare su un microscopio a fluorescenza standard. Questo approccio può essere applicato per controllare la vitalità degli sferoidi ed eseguire la standardizzazione, che è importante nella modellazione del tessuto 3D, del microambiente tumorale e della biofabbricazione (micro)tissutale di successo.

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Bioingegneria Numero 182 biofabbricazione bioprinting microscopia a fluorescenza ipossia metabolismo sonda O2-sensibile ossigenazione cellule staminali sferoidi
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