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Eine Schritt-für-Schritt-Methode zum Nachweisneutralisierender Antikörper gegen AAV mit einem kolorimetrischen zellbasierten Assay…
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Eine Schritt-für-Schritt-Methode zum Nachweis neutralisierender Antikörper gegen AAV mit einem kolorimetrischen zellbasierten Assay
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Article
DOI:
10.3791/63419-v
•
05:53 min
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December 7th, 2021
Eine Schritt-für-Schritt-Methode zum Nachweis neutralisierender Antikörper gegen AAV mit einem kolorimetrischen zellbasierten Assay
December 7th, 2021
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Sebastian Bass-Stringer1,2, Colleen J. Thomas2,3, Clive N. May3, Paul Gregorevic4, Kate L. Weeks1,5,6, Julie R. McMullen1,2,5,6,7
1Baker Heart and Diabetes Institute, 2Department of Physiology, Anatomy and Microbiology, La Trobe University, 3Florey Institute of Neuroscience and Mental Health, University of Melbourne, 4Department of Physiology, Centre for Muscle Research (CMR), The University of Melbourne, 5Department of Diabetes, Central Clinical School, Monash University, 6Baker Department of Cardiometabolic Health, The University of Melbourne, 7Department of Physiology and Department of Medicine Alfred Hospital, Monash University
Chapters
Summary
Please note that all translations are automatically generated.
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Ein umfassendes Laborprotokoll und ein Analyse-Workflow werden für einen schnellen, kostengünstigen und unkomplizierten kolorimetrischen zellbasierten Assay zur Erkennung neutralisierender Elemente gegen AAV6 beschrieben.