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JoVE Journal Bioengineering
Storskala, automatisert produksjon av fettavledede stamcellesfæroider for 3D-bioprinting
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Storskala, automatisert produksjon av fettavledede stamcellesfæroider for 3D-bioprinting

Article DOI: 10.3791/63430-v • 07:40 min • March 31st, 2022
March 31st, 2022 •
1,2,3, 1,2,4, 1,2,4, 1,2, 2,3,4,5, 1,2,3,4
1Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro, 2Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology (Inmetro), 3Post-graduation Program of Translational Biomedicine (Biotrans), Unigranrio, 4Post-graduation Program in Biotechnology, National Institute of Metrology, Quality and Technology (Inmetro), 5Dental School, Fluminense Federal Fluminense

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Her beskriver vi storskala produksjon av fettavledet stromal/ stamcelle (ASC) sfæroider ved hjelp av et automatisert pipetteringssystem for å frø cellesuspensjonen, og dermed sikre homogenitet av sfæroid størrelse og form. Disse ASC-sfæroidene kan brukes som byggesteiner for 3D-bioprintingsmetoder.

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Bioteknologi utgave 181
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