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Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacuna
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Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacuna

Article DOI: 10.3791/63470-v • 11:47 min • February 1st, 2022
February 1st, 2022 •
1, 1, 1, 1, 2, 2, 3, 3, 1
1Botanical Institute, Karlsruhe Institute of Technology KIT, 2Institute for Biological Interfaces 5 (IBG 5), Karlsruhe Institute of Technology KIT, 3Institut für Technische Mikrobiologie, Hochschule Mannheim

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Summary
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Phormidium lacuna is a filamentous cyanobacterium that was isolated from marine rockpools. This article describes the isolation of filaments from natural sources, DNA extraction, genome sequencing, natural transformation, expression of sfGFP, cryoconservation, and motility methods.

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Natural Transformation Protein Expression Cryoconservation Filamentous Cyanobacterium Phormidium Lacuna Oscillatoriales DNA Cells Growth Medium Transformation Technique Motion Studies Ultrasound Treatment Culture Inoculation Liquid F2 Medium P.lacuna Filaments White Light Agitation Optical Density Centrifuge Supernatant Pellet Suspension F2+Bacto Agar Plates
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