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Naturlig transformation, proteinuttryck och kryokonservering av den trådformiga cyanobakterien Phormidium lacuna
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Naturlig transformation, proteinuttryck och kryokonservering av den trådformiga cyanobakterien Phormidium lacuna

Article DOI: 10.3791/63470-v • 11:47 min • February 1st, 2022
February 1st, 2022 •
1, 1, 1, 1, 2, 2, 3, 3, 1
1Botanical Institute, Karlsruhe Institute of Technology KIT, 2Institute for Biological Interfaces 5 (IBG 5), Karlsruhe Institute of Technology KIT, 3Institut für Technische Mikrobiologie, Hochschule Mannheim

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Phormidium lacuna är en trådformig cyanobakterie som isolerades från marina stenpooler. Denna artikel beskriver isolering av filament från naturliga källor, DNA-extraktion, genomsekvensering, naturlig transformation, uttryck av sfGFP, kryokonservation och motilitetsmetoder.

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Biologi utgåva 180
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