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Journal / Biology / Filamentöz Cyanobacterium Phormidium lacuna'nın…
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Filamentöz Cyanobacterium Phormidium lacuna'nın Doğal Transformasyonu, Protein İfadesi ve Kriyokorunumu
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Filamentöz Cyanobacterium Phormidium lacuna'nın Doğal Transformasyonu, Protein İfadesi ve Kriyokorunumu

Article DOI: 10.3791/63470-v • 11:47 min • February 1st, 2022
February 1st, 2022 •
1, 1, 1, 1, 2, 2, 3, 3, 1
1Botanical Institute, Karlsruhe Institute of Technology KIT, 2Institute for Biological Interfaces 5 (IBG 5), Karlsruhe Institute of Technology KIT, 3Institut für Technische Mikrobiologie, Hochschule Mannheim

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Phormidium lacuna , deniz kaya havuzlarından izole edilmiş filamentli bir siyanobakteridir. Bu makalede filamentlerin doğal kaynaklardan izolasyonu, DNA ekstraksiyonu, genom dizilimi, doğal transformasyon, sfGFP ekspresyonu, kriyokonservasyon ve motiliteye yöntemleri anlatılmaktadır.

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