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Journal / Biochemistry / 使用场效应生物传感技术探索分子伴侣Hsp90与其客户蛋白激酶Cdc37之间的生物分子相互作用…
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Biochemistry

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JoVE Journal Biochemistry
使用场效应生物传感技术探索分子伴侣Hsp90与其客户蛋白激酶Cdc37之间的生物分子相互作用
Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions
Journal (Biochemistry)
Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions
Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
Journal (Biochemistry)
Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
 
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使用场效应生物传感技术探索分子伴侣Hsp90与其客户蛋白激酶Cdc37之间的生物分子相互作用

Article DOI: 10.3791/63495-v • 09:39 min • March 31st, 2022
March 31st, 2022 •
*1, *1, 2, 2, 1
1The Azrieli Faculty of Medicine in the Galilee, Bar-Ilan University, 2Asian Liver Center, Department of Surgery, Stanford University School of Medicine
* These authors contributed equally

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Summary
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场效应生物传感（FEB）是一种用于检测生物分子相互作用的无标记技术。它测量通过石墨烯生物传感器的电流，结合目标被固定到该传感器。FEB技术用于评估Hsp90和Cdc37之间的生物分子相互作用，并检测到两种蛋白质之间的强相互作用。

Tags

生物化学，第181期，细胞分裂周期37，Cdc37，热休克蛋白90，Hsp90，蛋白激酶，石墨烯生物传感器，场效应生物传感，癌症，生物分子相互作用
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