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Organische eiwitprecipitatie op basis van oplosmiddelen voor robuuste proteoomzuivering vóór massaspectrometrie
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Organische eiwitprecipitatie op basis van oplosmiddelen voor robuuste proteoomzuivering vóór massaspectrometrie

Article DOI: 10.3791/63503-v • 11:12 min • February 7th, 2022
February 7th, 2022 •
1, 1, 1, 2, 2, 1
1Department of Chemistry, Dalhousie University, 2Proteoform Scientific Inc.

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Het huidige protocol beschrijft eiwitprecipitatie op basis van oplosmiddelen onder gecontroleerde omstandigheden voor robuust en snel herstel en zuivering van proteoommonsters voorafgaand aan massaspectrometrie.

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Chemie Nummer 180 Massaspectrometrie proteomics monstervoorbereiding natriumdodecylsulfaat precipitatie eiwitvertering aceton chloroform/methanol/water
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