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Cancer Research
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实时检测和捕获共培养物中的侵袭细胞亚群
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Real-Time Detection and Capture of Invasive Cell Subpopulations from Co-Cultures
Please note that all translations are automatically generated.
Click here for the English version.
实时检测和捕获共培养物中的侵袭细胞亚群
DOI:
10.3791/63512-v
•
08:00 min
•
March 30, 2022
•
Ghada M. Sharif
,
Leon Der
,
Anna T. Riegel
,
Makarand Paranjape
,
Anton Wellstein
1
Lombardi Comprehensive Cancer Center
,
Georgetown University Medical Center
,
2
Department of Physics
,
Georgetown University
Chapters
00:05
Introduction
00:49
Cell Culture
01:17
Cell Seeding and Assembly
04:09
Impedance Measurement
04:44
Detachment and Cell Collection
05:54
Results: Detection and Capture of Invasive Cell Subpopulations in Real-Time
07:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种实时检测和捕获侵袭性细胞亚群的方法。实验设计使用实时细胞分析,通过监测细胞电阻抗的变化。可以捕获复杂组织中的浸润性癌症,免疫,内皮或基质细胞,并且可以评估共培养物的影响。
Tags
Real-time Detection
Invasive Cell Subpopulations
Co-cultures
Cell Invasion
Cell Motility
Cell Culture
Cell Analyzer
Cell Recovery
Cell Therapy
Metastasis
Cancer Cells
Stromal Cells
Immune Cells
Heterogeneous Cell Mixes
Trypsin
EDTA
Serum
Cell Analysis
Cell Migration
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