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Standaardisatie en onderhoud van 3D canine hepatische en intestinale organoïde culturen voor gebruik in biomedisch onderzoek
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Standaardisatie en onderhoud van 3D canine hepatische en intestinale organoïde culturen voor gebruik in biomedisch onderzoek

Article DOI: 10.3791/63515-v • 08:39 min • January 31st, 2022
January 31st, 2022 •
1, 1, 2, 3, 2, 2, 4, 4, 1,3, 2,3
1Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, 33D Health Solutions Inc., 4Department of Molecular Virology and Microbiology, Baylor College of Medicine

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Experimentele methoden om volwassen stamcellen uit intestinale en leverweefsels van honden te oogsten om 3D-organoïde culturen vast te stellen, worden beschreven. Bovendien worden de laboratoriumtechnieken besproken om een consistente groei te garanderen en standaard operationele procedures te bieden om intestinale en hepatische organoïdeculturen van honden te oogsten, biobank en nieuw leven in te blazen.

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Geneeskunde Nummer 179 Organoïde hond hond stamcel lever darm One Health standaardisatie
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