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Journal / Medicine / Standardizzazione e mantenimento di colture…
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JoVE Journal Medicine
Standardizzazione e mantenimento di colture organoidi epatiche e intestinali canine 3D per l'uso nella ricerca biomedica
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Standardizzazione e mantenimento di colture organoidi epatiche e intestinali canine 3D per l'uso nella ricerca biomedica

Article DOI: 10.3791/63515-v • 08:39 min • January 31st, 2022
January 31st, 2022 •
1, 1, 2, 3, 2, 2, 4, 4, 1,3, 2,3
1Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, 33D Health Solutions Inc., 4Department of Molecular Virology and Microbiology, Baylor College of Medicine

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Vengono descritti metodi sperimentali per raccogliere cellule staminali adulte da tessuti intestinali ed epatici canini per stabilire colture organoidi 3D. Inoltre, vengono discusse le tecniche di laboratorio per garantire una crescita costante e fornire procedure operative standard per raccogliere, biobancare e far rivivere colture organoidi intestinali ed epatiche canine.

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Medicina Numero 179 Organoide cane cane cellule staminali fegato intestino One Health standardizzazione
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